A Rapid and Specific Assay for the Detection of MERS-CoV
Identifieur interne : 000A93 ( Main/Exploration ); précédent : 000A92; suivant : 000A94A Rapid and Specific Assay for the Detection of MERS-CoV
Auteurs : Pei Huang [République populaire de Chine] ; Hualei Wang [République populaire de Chine] ; Zengguo Cao [République populaire de Chine] ; Hongli Jin [République populaire de Chine] ; Hang Chi [République populaire de Chine] ; Jincun Zhao [République populaire de Chine] ; Beibei Yu [République populaire de Chine] ; Feihu Yan [République populaire de Chine] ; Xingxing Hu [République populaire de Chine] ; Fangfang Wu [République populaire de Chine] ; Cuicui Jiao [République populaire de Chine] ; Pengfei Hou [République populaire de Chine] ; Shengnan Xu [République populaire de Chine] ; Yongkun Zhao [République populaire de Chine] ; Na Feng [République populaire de Chine] ; Jianzhong Wang [République populaire de Chine] ; Weiyang Sun [République populaire de Chine] ; Tiecheng Wang [République populaire de Chine] ; Yuwei Gao [République populaire de Chine] ; Songtao Yang [République populaire de Chine] ; Xianzhu Xia [République populaire de Chine]Source :
- Frontiers in Microbiology [ 1664-302X ] ; 2018.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human coronavirus that can cause human respiratory disease. The development of a detection method for this virus that can lead to rapid and accurate diagnosis would be significant. In this study, we established a nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV. The RT-LAMP-VF assay was performed in a constant temperature water bath for 30 min, and the result was visible by the naked eye within 5 min. The RT-LAMP-VF assay was capable of detecting 2 × 101 copies/μl of synthesized RNA transcript and 1 × 101 copies/μl of MERS-CoV RNA. The method exhibits no cross-reactivities with multiple CoVs including SARS-related (SARSr)-CoV, HKU4, HKU1, OC43 and 229E, and thus exhibits high specificity. Compared to the real-time RT-PCR (rRT-PCR) method recommended by the World Health Organization (WHO), the RT-LAMP-VF assay is easy to handle, does not require expensive equipment and can rapidly complete detection within 35 min.
Url:
DOI: 10.3389/fmicb.2018.01101
PubMed: 29896174
PubMed Central: 5987675
Affiliations:
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University</institution>,<addr-line>Guangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><institution>Guangzhou Eighth People’s Hospital of Guangzhou Medical University</institution>,<addr-line>Guangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Beibei" sort="Yu, Beibei" uniqKey="Yu B" first="Beibei" last="Yu">Beibei Yu</name><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Department of Clinical Laboratory, College of Medicine, Sir Run Run Shaw Hospital, Zhejiang University</institution>,<addr-line>Hangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yan, Feihu" sort="Yan, Feihu" uniqKey="Yan F" first="Feihu" last="Yan">Feihu Yan</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hu, Xingxing" sort="Hu, Xingxing" uniqKey="Hu X" first="Xingxing" last="Hu">Xingxing Hu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Animal Science and Technology College, Jilin Agricultural University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country 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populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Jiao, Cuicui" sort="Jiao, Cuicui" uniqKey="Jiao C" first="Cuicui" last="Jiao">Cuicui Jiao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hou, Pengfei" sort="Hou, Pengfei" uniqKey="Hou P" first="Pengfei" last="Hou">Pengfei Hou</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>College of Veterinary Medicine, Jilin University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Shengnan" sort="Xu, Shengnan" uniqKey="Xu S" first="Shengnan" last="Xu">Shengnan Xu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Animal Science and Technology College, Jilin Agricultural University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Feng, Na" sort="Feng, Na" uniqKey="Feng N" first="Na" last="Feng">Na Feng</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation 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id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29896174</idno><idno type="pmc">5987675</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987675</idno><idno type="RBID">PMC:5987675</idno><idno type="doi">10.3389/fmicb.2018.01101</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000C81</idno><idno 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Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>College of Veterinary Medicine, Jilin University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Cao, Zengguo" sort="Cao, Zengguo" uniqKey="Cao Z" first="Zengguo" last="Cao">Zengguo Cao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>College of Veterinary Medicine, Jilin University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Jin, Hongli" sort="Jin, Hongli" uniqKey="Jin H" first="Hongli" last="Jin">Hongli Jin</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>College of Veterinary Medicine, Jilin University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chi, Hang" sort="Chi, Hang" uniqKey="Chi H" first="Hang" last="Chi">Hang Chi</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Heath, The First Affiliated Hospital of Guangzhou Medical University</institution>,<addr-line>Guangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><institution>Guangzhou Eighth People’s Hospital of Guangzhou Medical University</institution>,<addr-line>Guangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Beibei" sort="Yu, Beibei" uniqKey="Yu B" first="Beibei" last="Yu">Beibei Yu</name><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Department of Clinical Laboratory, College of Medicine, Sir Run Run Shaw Hospital, Zhejiang University</institution>,<addr-line>Hangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yan, Feihu" sort="Yan, Feihu" uniqKey="Yan F" first="Feihu" last="Yan">Feihu Yan</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hu, Xingxing" sort="Hu, Xingxing" uniqKey="Hu X" first="Xingxing" last="Hu">Xingxing Hu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Animal Science and Technology College, Jilin Agricultural University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Fangfang" sort="Wu, Fangfang" uniqKey="Wu F" first="Fangfang" last="Wu">Fangfang Wu</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Jiao, Cuicui" sort="Jiao, Cuicui" uniqKey="Jiao C" first="Cuicui" last="Jiao">Cuicui Jiao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hou, Pengfei" sort="Hou, Pengfei" uniqKey="Hou P" first="Pengfei" last="Hou">Pengfei Hou</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>College of Veterinary Medicine, Jilin University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Shengnan" sort="Xu, Shengnan" uniqKey="Xu S" first="Shengnan" last="Xu">Shengnan Xu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Animal Science and Technology College, Jilin Agricultural University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Feng, Na" sort="Feng, Na" uniqKey="Feng N" first="Na" last="Feng">Na Feng</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Jianzhong" sort="Wang, Jianzhong" uniqKey="Wang J" first="Jianzhong" last="Wang">Jianzhong Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Animal Science and Technology College, Jilin Agricultural University</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Weiyang" sort="Sun, Weiyang" uniqKey="Sun W" first="Weiyang" last="Sun">Weiyang Sun</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Tiecheng" sort="Wang, Tiecheng" uniqKey="Wang T" first="Tiecheng" last="Wang">Tiecheng Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences</institution>,<addr-line>Changchun</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses</institution>,<addr-line>Yangzhou</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Frontiers in Microbiology</title><idno type="eISSN">1664-302X</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human coronavirus that can cause human respiratory disease. The development of a detection method for this virus that can lead to rapid and accurate diagnosis would be significant. In this study, we established a nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV. The RT-LAMP-VF assay was performed in a constant temperature water bath for 30 min, and the result was visible by the naked eye within 5 min. The RT-LAMP-VF assay was capable of detecting 2 × 10<sup>1</sup> copies/μl of synthesized RNA transcript and 1 × 10<sup>1</sup> copies/μl of MERS-CoV RNA. The method exhibits no cross-reactivities with multiple CoVs including SARS-related (SARSr)-CoV, HKU4, HKU1, OC43 and 229E, and thus exhibits high specificity. Compared to the real-time RT-PCR (rRT-PCR) method recommended by the World Health Organization (WHO), the RT-LAMP-VF assay is easy to handle, does not require expensive equipment and can rapidly complete detection within 35 min.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Azhar, E I" uniqKey="Azhar E">E. I. Azhar</name></author><author><name sortKey="El Kafrawy, S A" uniqKey="El Kafrawy S">S. A. El-Kafrawy</name></author><author><name sortKey="Farraj, S A" uniqKey="Farraj S">S. A. Farraj</name></author><author><name sortKey="Hassan, A M" uniqKey="Hassan A">A. M. Hassan</name></author><author><name sortKey="Al Saeed, M S" uniqKey="Al Saeed M">M. S. Al-Saeed</name></author><author><name sortKey="Hashem, A M" uniqKey="Hashem A">A. M. Hashem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bhadra, S" uniqKey="Bhadra S">S. 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Fouchier</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Pei" sort="Huang, Pei" uniqKey="Huang P" first="Pei" last="Huang">Pei Huang</name></noRegion><name sortKey="Cao, Zengguo" sort="Cao, Zengguo" uniqKey="Cao Z" first="Zengguo" last="Cao">Zengguo Cao</name><name sortKey="Cao, Zengguo" sort="Cao, Zengguo" uniqKey="Cao Z" first="Zengguo" last="Cao">Zengguo Cao</name><name sortKey="Chi, Hang" sort="Chi, Hang" uniqKey="Chi H" first="Hang" last="Chi">Hang Chi</name><name sortKey="Feng, Na" sort="Feng, Na" uniqKey="Feng N" first="Na" last="Feng">Na Feng</name><name sortKey="Feng, Na" sort="Feng, Na" uniqKey="Feng N" first="Na" last="Feng">Na Feng</name><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><name sortKey="Hou, Pengfei" sort="Hou, Pengfei" uniqKey="Hou P" first="Pengfei" last="Hou">Pengfei Hou</name><name sortKey="Hou, Pengfei" sort="Hou, Pengfei" uniqKey="Hou P" first="Pengfei" last="Hou">Pengfei Hou</name><name sortKey="Hu, Xingxing" sort="Hu, Xingxing" uniqKey="Hu X" first="Xingxing" last="Hu">Xingxing Hu</name><name sortKey="Hu, Xingxing" sort="Hu, Xingxing" uniqKey="Hu X" first="Xingxing" last="Hu">Xingxing Hu</name><name sortKey="Huang, Pei" sort="Huang, Pei" uniqKey="Huang P" first="Pei" last="Huang">Pei Huang</name><name sortKey="Jiao, Cuicui" sort="Jiao, Cuicui" uniqKey="Jiao C" first="Cuicui" last="Jiao">Cuicui Jiao</name><name sortKey="Jin, Hongli" sort="Jin, Hongli" uniqKey="Jin H" first="Hongli" last="Jin">Hongli Jin</name><name sortKey="Jin, Hongli" sort="Jin, Hongli" uniqKey="Jin H" first="Hongli" last="Jin">Hongli Jin</name><name sortKey="Sun, Weiyang" sort="Sun, Weiyang" uniqKey="Sun W" first="Weiyang" last="Sun">Weiyang Sun</name><name sortKey="Sun, Weiyang" sort="Sun, Weiyang" uniqKey="Sun W" first="Weiyang" last="Sun">Weiyang Sun</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Jianzhong" sort="Wang, Jianzhong" uniqKey="Wang J" first="Jianzhong" last="Wang">Jianzhong Wang</name><name sortKey="Wang, Tiecheng" sort="Wang, Tiecheng" uniqKey="Wang T" first="Tiecheng" last="Wang">Tiecheng Wang</name><name sortKey="Wang, Tiecheng" sort="Wang, Tiecheng" uniqKey="Wang T" first="Tiecheng" last="Wang">Tiecheng Wang</name><name sortKey="Wu, Fangfang" sort="Wu, Fangfang" uniqKey="Wu F" first="Fangfang" last="Wu">Fangfang Wu</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Xu, Shengnan" sort="Xu, Shengnan" uniqKey="Xu S" first="Shengnan" last="Xu">Shengnan Xu</name><name sortKey="Xu, Shengnan" sort="Xu, Shengnan" uniqKey="Xu S" first="Shengnan" last="Xu">Shengnan Xu</name><name sortKey="Yan, Feihu" sort="Yan, Feihu" uniqKey="Yan F" first="Feihu" last="Yan">Feihu Yan</name><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><name sortKey="Yu, Beibei" sort="Yu, Beibei" uniqKey="Yu B" first="Beibei" last="Yu">Beibei Yu</name><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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